Li WANG,Yong WEI,Guiping YUAN,et al.Molecular Characterization and Virulence Genes of Aeromonas hydrophila Isolated from the Chinese Giant Salamander (Andrias davidianus)[J].Asian Herpetological Research(AHR),2012,3(4):303-309.[doi:10.3724/SP.J.1245.2012.00303]
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Molecular Characterization and Virulence Genes of Aeromonas hydrophila Isolated from the Chinese Giant Salamander (Andrias davidianus)
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2012 VoI.3 No.4
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Original Article
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Molecular Characterization and Virulence Genes of Aeromonas hydrophila Isolated from the Chinese Giant Salamander (Andrias davidianus)
Li WANG1 Yong WEI2* Guiping YUAN3 Min DAI4 and Xueliang CHEN5
1 Key Laboratory of Animal Genetics and Breeding of State Ethnic Affairs Commission & Ministry of Education, College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, Sichuan, China
2 Sichuan Academy of Animal Sciences, Chengdu 610066, Sichuan, China
3 Analytical & Testing Center, Sichuan University, Chengdu 610064, Sichuan, China
4 School of Laboratory Medicine, Chengdu Medical College, Chengdu 610083, Sichuan, China
5 Xiamen Sea World, Xiamen 361002, Fujian, China
Chinese giant salamander 16S rDNA virulence gene drug resistance Aeromonas hydrophila
The Chinese giant salamander (Andrias davidianus) is the largest living amphibian in the world. Aeromonas hydrophila strain L602 was isolated from A. davidianus. The 16S rDNA gene of this isolate was amplified using PCR, and the phylogenetic tree was constructed by the neighbor-joining method. Four virulence genes (aerA, aha1, hly and alt) of A. hydrophila were amplified by PCR and drug resistances were tested using Kirby-Bauer disk diffusion method. The results showed that the length of this 16S rDNA sequence was 1453 bp, which showed 99% homology with A. hydrophila. The GenBank accession number was JX155398. Phylogenetic analysis indicated it grouped together with A. hydrophila. Four virulence genes were all detected, indicating that strain L602 was highly virulent. This stain was resistant to four antibiotics (vibramycin, furazolidone, ampicillin and erythromycin), while it was insensitive to streptomycin. Furthermore, this strain was susceptible to six antibiotics (sulfafurazole, ciprofloxacin, penbritin, norfloxacin, florfenicol and enrofloxacin). This study will help to validate the classification and virulence of pathogenic bacteria in amphibians.


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